| City Name | Population | State | Rank |
|---|---|---|---|
| Kansas City | 459,787 | Missouri | 1 |
| St. Louis City | 319,294 | Missouri | 2 |
| Springfield City | 159,498 | Missouri | 3 |
| Independence City | 116,830 | Missouri | 4 |
| Columbia City | 108,500 | Missouri | 5 |
| Lee's Summit City | 91,364 | Missouri | 6 |
| O'fallon City | 79,329 | Missouri | 7 |
| St. Joseph City | 76,780 | Missouri | 8 |
| St. Charles City | 65,794 | Missouri | 9 |
| St. Peters City | 52,575 | Missouri | 10 |
| Blue Springs City | 52,575 | Missouri | 11 |
| Florissant City | 52,158 | Missouri | 12 |
| Joplin City | 50,150 | Missouri | 13 |
| Chesterfield City | 47,484 | Missouri | 14 |
| Jefferson City | 43,079 | Missouri | 15 |
| Cape Girardeau City | 37,941 | Missouri | 16 |
| Oakville | 36,143 | Missouri | 17 |
| Wildwood City | 35,517 | Missouri | 18 |
| University City | 35,371 | Missouri | 19 |
| Ballwin City | 30,404 | Missouri | 20 |
| Raytown City | 29,526 | Missouri | 21 |
| Liberty City | 29,149 | Missouri | 22 |
| Wentzville City | 29,070 | Missouri | 23 |
| Mehlville | 28,380 | Missouri | 24 |
| Kirkwood City | 27,540 | Missouri | 25 |
| Maryland Heights City | 27,472 | Missouri | 26 |
| Hazelwood City | 25,703 | Missouri | 27 |
| Gladstone City | 25,410 | Missouri | 28 |
| Grandview City | 24,475 | Missouri | 29 |
| Belton City | 23,116 | Missouri | 30 |
| Webster Groves City | 22,995 | Missouri | 31 |
| Sedalia City | 21,387 | Missouri | 32 |
| Ferguson City | 21,203 | Missouri | 33 |
| Arnold City | 20,808 | Missouri | 34 |
| Affton | 20,307 | Missouri | 35 |
| Spanish Lake | 19,650 | Missouri | 36 |
| Rolla City | 19,559 | Missouri | 37 |
| Raymore City | 19,206 | Missouri | 38 |
| Old Jamestown | 19,184 | Missouri | 39 |
| Nixa City | 19,022 | Missouri | 40 |
| Warrensburg City | 18,838 | Missouri | 41 |
| Manchester City | 18,094 | Missouri | 42 |
| Hannibal City | 17,916 | Missouri | 43 |
| Creve Coeur City | 17,833 | Missouri | 44 |
| Ozark City | 17,820 | Missouri | 45 |
| Kirksville City | 17,505 | Missouri | 46 |
| Poplar Bluff City | 17,023 | Missouri | 47 |
| Lemay | 16,645 | Missouri | 48 |
| Concord | 16,421 | Missouri | 49 |
| Sikeston City | 16,318 | Missouri | 50 |
| Farmington City | 16,240 | Missouri | 51 |
| Overland City | 16,062 | Missouri | 52 |
| Clayton City | 15,939 | Missouri | 53 |
| Fort Leonard Wood | 15,061 | Missouri | 54 |
| Republic City | 14,751 | Missouri | 55 |
| Jennings City | 14,712 | Missouri | 56 |
| Lake St. Louis City | 14,545 | Missouri | 57 |
| Lebanon City | 14,474 | Missouri | 58 |
| Carthage City | 14,378 | Missouri | 59 |
| Washington City | 13,982 | Missouri | 60 |
| Moberly City | 13,974 | Missouri | 61 |
| Jackson City | 13,758 | Missouri | 62 |
| Marshall City | 13,065 | Missouri | 63 |
| St. Ann City | 13,020 | Missouri | 64 |
| Grain Valley City | 12,854 | Missouri | 65 |
| Fulton City | 12,790 | Missouri | 66 |
| West Plains City | 11,986 | Missouri | 67 |
| Maryville City | 11,972 | Missouri | 68 |
| Crestwood City | 11,912 | Missouri | 69 |
| Neosho City | 11,835 | Missouri | 70 |
| Festus City | 11,602 | Missouri | 71 |
| Bridgeton City | 11,550 | Missouri | 72 |
| Mexico City | 11,543 | Missouri | 73 |
| Dardenne Prairie City | 11,494 | Missouri | 74 |
| Excelsior Springs City | 11,084 | Missouri | 75 |
| Webb City | 10,996 | Missouri | 76 |
| Kennett City | 10,932 | Missouri | 77 |
| Bellefontaine Neighbors City | 10,860 | Missouri | 78 |
| Town And Country City | 10,815 | Missouri | 79 |
| Troy City | 10,540 | Missouri | 80 |
| Branson City | 10,520 | Missouri | 81 |
| Bolivar City | 10,325 | Missouri | 82 |
| Union City | 10,204 | Missouri | 83 |
| Eureka City | 10,189 | Missouri | 84 |
| Harrisonville City | 10,019 | Missouri | 85 |
| Cameron City | 9,933 | Missouri | 86 |
| Chillicothe City | 9,515 | Missouri | 87 |
| Ellisville City | 9,133 | Missouri | 88 |
| Clinton City | 9,008 | Missouri | 89 |
| Berkeley City | 8,978 | Missouri | 90 |
| Monett City | 8,873 | Missouri | 91 |
| Park Hills City | 8,759 | Missouri | 92 |
| Murphy | 8,690 | Missouri | 93 |
| Richmond Heights City | 8,603 | Missouri | 94 |
| Ladue City | 8,521 | Missouri | 95 |
| Sunset Hills City | 8,496 | Missouri | 96 |
| Smithville City | 8,425 | Missouri | 97 |
| Nevada City | 8,386 | Missouri | 98 |
| Kearney City | 8,381 | Missouri | 99 |
| Des Peres City | 8,373 | Missouri | 100 |
| Boonville City | 8,319 | Missouri | 101 |
| Perryville City | 8,225 | Missouri | 102 |
| Pleasant Hill City | 8,113 | Missouri | 103 |
| Brentwood City | 8,055 | Missouri | 104 |
| Maplewood City | 8,046 | Missouri | 105 |
| Warrenton City | 7,880 | Missouri | 106 |
| Dexter City | 7,864 | Missouri | 107 |
| Oak Grove City | 7,795 | Missouri | 108 |
| Olivette City | 7,737 | Missouri | 109 |
| Sappington | 7,580 | Missouri | 110 |
| Aurora City | 7,508 | Missouri | 111 |
| Carl Junction City | 7,445 | Missouri | 112 |
| Sullivan City | 7,081 | Missouri | 113 |
| Pacific City | 7,002 | Missouri | 114 |
| Valley Park City | 6,942 | Missouri | 115 |
| Black Jack City | 6,929 | Missouri | 116 |
| Bonne Terre City | 6,864 | Missouri | 117 |
| Marshfield City | 6,633 | Missouri | 118 |
| St. John City | 6,517 | Missouri | 119 |
| De Soto City | 6,400 | Missouri | 120 |
| Shrewsbury City | 6,254 | Missouri | 121 |
| Caruthersville City | 6,168 | Missouri | 122 |
| Trenton City | 6,001 | Missouri | 123 |
| Charleston City | 5,947 | Missouri | 124 |
| Glendale City | 5,925 | Missouri | 125 |
| Richmond City | 5,797 | Missouri | 126 |
| Barnhart | 5,682 | Missouri | 127 |
| Battlefield City | 5,590 | Missouri | 128 |
| Parkville City | 5,554 | Missouri | 129 |
| Pevely City | 5,484 | Missouri | 130 |
| Macon City | 5,471 | Missouri | 131 |
| Weldon Spring City | 5,443 | Missouri | 132 |
| Glasgow Village | 5,429 | Missouri | 133 |
| Bowling Green City | 5,334 | Missouri | 134 |
| Odessa City | 5,300 | Missouri | 135 |
| Willard City | 5,288 | Missouri | 136 |
| Greenwood City | 5,221 | Missouri | 137 |
| Savannah City | 5,057 | Missouri | 138 |
| Desloge City | 5,054 | Missouri | 139 |
| Dellwood City | 5,025 | Missouri | 140 |
| Normandy City | 5,008 | Missouri | 141 |